5-ROX NHS Ester

Catalog#
Unit Size
Price (USD)
Availability
Qty
Cat.#: 1274-25
Unit size: 25 mg
$435.00
in stock
Cat.#: 1274-100
Unit size: 100 mg
$795.00
in stock
Cat.#: 1274-1000
Unit size: 1000 mg
$2,250.00
in stock

5-Carboxy-X-rhodamine N-succinimidyl ester (5-ROX NHS Ester) is used as an amine coupling reagent to form 5-carboxy-X-rhodamine (ROX) derivatized compounds such as proteins, nucleic acids and drugs that may be analyzed by fluorescence resonance energy transfer (FRET) and fluorescence quenching applications. Labeling reagent for preparation of charge-modified dye-labeled ddNTPs to “direct-load” DNA sequencing. Conjugates of this dye have longer-wavelength spectra than the spectra of Lisaamine™ rhodamine B conjugates, but somewhat shorter-wavelength spectra than those of Texas Red™ conjugates.

Reactivity
Primary amine
Abs/Em Maxima
582/606 nm
Extinction coefficient
93,000 cm-1M-1
Solubility
DMSO, DMF, MeOH
Spectrally similar dyes
Alexa Fluor® 568, TAMRA, CF™ 568
CAS
209734-74-7
Molecular weight
631.69
Storage conditions
-20C
Shipping conditions
Ambient temperature

spectrum

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