AZDye 532 Maleimide

Catalog#

Unit Size

Price (USD)

Availability

Qty

Cat.#: 1042-1

Unit size: 1 mg

$169.00

in stock

Cat.#: 1042-5

Unit size: 5 mg

$565.00

in stock

Cat.#: 1042-25

Unit size: 25 mg

$1,595.00

in stock

Cat.#: 1042-100

Unit size: 100 mg

$2,950.00

in stock

AZDye™ 532 Maleimide is a water-soluble, photostable, bright yellow-fluorescent dye with pH insensitive emission from pH 4 to pH 10. The excitation of AZDye™ 532 is ideally suited for the frequency-doubled Nd:YAG laser line. AZDye™ 532 dye can be conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates, and is often used for the generation of stable signals in imaging and flow cytometry.

Maleimide is the most popular sulfhydryl-reactive group for conjugating the dye to a thiol group on a protein, oligonucleotide thiophosphate, or low molecular weight ligand. The maleimide group specifically and efficiently reacts with reduced thiols (sulfhydryl groups, –SH) at pH 6.5 to 7.5 to form a stable thioether bond. The resulting conjugates exhibit brighter fluorescence and greater photostability than the conjugates of many other spectrally similar fluorophores.

AZDye™ 532 Maleimide dye is structurally identical to Alexa Fluor™ 532 C5 Maleimide.

Specifications
Structure
Abs/Em Spectra
Protein Labeling Calculator
Documents

Reactivity

Thiol (Cys)

Abs/Em Maxima

530/555 nm

Extinction coefficient

78,000 cm-1M-1

Solubility

Water, DMSO, DMF

Spectrally similar dyes

Alexa Fluor® 532, DyLight® 532

Molecular weight

790.90 (protonated)

Storage conditions

-20C

Shipping conditions

Ambient temperature

Protein name

Protein molecular weight (Da)

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Volume of protein to be labeled (µL)

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Volume of DMSO required to make a stock solution (µL)

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Amount of DMSO in labeling reaction (%)